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Cell lysates were centrifuged at 17,000 g and 4 C for 10 minutes, and supernatants added to 40 L anti-FLAG M2 agarose beads (A2220, Sigma-Aldrich) for overnight rotating incubation at 4 C to increase binding efficiency

10.3109/00498254.2015.1129080 Xenobiotica 154 ZouD.LouJ.KeJ.MeiS.LiJ.GongY.et al (2018)